ĐẦU LỌC SẮC KÝ
Hãng sản xuất: Vertical – Thái Lan
Có 4 điểm chính để chọn đầu lọc cho phù hợp
- Khả năng chịu áp suất
- Thể tích mẫu
| Size | Burst Pressure | Retain Volume | Sample Volume |
| 4mm | 75psi | 10uL | ≤ 2mL |
| 13mm | 100psi | 30uL | ≤ 10mL |
| 17mm | 95psi | 45uL | ≤ 30mL |
| 25mm | 80psi | 125uL | ≤ 150mL |
- Loại vật liệu lọc
Có các vật liệu lọc như sau
| Membrane Type Applications | Ứng dụng |
| Nylon Nylon (Polyamide) is hydrophilic, good solvent resistance and medium protein binding. |
– Filtration of all aqueous and most solvent based samples – Use with care for biological compounds |
| PTFE PTFE (Polytetrafl uoroethylene) is hydrophobic, highest solvent resistance and high protein binding. |
– Filtration of non-aqueous or solvent based samples – Good for filtering and degassing chromatography |
| PVDF-HL, hydrophilic Hydrophilic PVDF (Polyvinylidene difl uoride) is high solvent resistance, low protein binding and good flow rate characteristics. |
– Filtration of all aqueous and most solvent based samples – General filtration of biological samples |
| PVDF-HB, hydrophobic Hydrophobic PVDF (Polyvinylidene Fluoride) is high solvent resistance, low protein binding and good fl ow rate characteristics. |
– Filtration of non-aqueous and most solvent based samples – General filtration of biological samples |
| CA CA (Cellulose Acetate) is hydrophilic, limited solvent resistance and very low protein binding. |
– Ideal for aqueous based samples – Ideal for tissue culture and sensitive biological samples |
| RC RC (Regenerated Cellulose) is hydrophilic, high solvent resistance and lowest protein binding. |
– Ideal for aqueous based and organic samples – General fi ltration of biological samples |
| MCE MCE (Mixed Cellulose Ester) is hydrophilic, limit solvent resistance, high protein binding and excellent fl ow rate characteristics. |
– Ideal for aqueous based samples – Specifically filtration of trace protein contamination |
| PP PP (Polypropylene) is hydrophobic, high solvent resistance and lower protein binding than PVDF. |
– Filtration of all aqueous and most solvent based samples – General filtration of biological samples |
| PES PES (Polyethersulfone) is hydrophilic, limited solvent resistance, low protein binding and good fl ow rate characteristics. |
– Ideal for aqueous based samples – Ideal for tissue culture and sensitive biological samples |
| GMF GMF (Glass microfiber) is commonly used as pre-fi lters to remove large particulates and minimized sample loss. |
– General filtration of biological samples – Used in a variety of sample clean-up and prefiltration |
- Loại dung môi
| Dung môi | NYLON | PTFE | PVDF-HL | PP | PES | CA | RC | MCE |
| Acids | ||||||||
| Acetic, Glacial | L | C | C | C | C | N | C | N |
| Acetic, 25% | C | C | C | C | C | C | C | N |
| Formic, 25% | N | C | C | C | C | L | C | – |
| Hydrochloric, 25% | N | C | C | C | C | N | N | L |
| Nitric, 25% | N | C | C | C | L | N | N | N |
| Phosphoric, 25% | N | C | C | C | C | C | L | N |
| Sulfuric, 25% | N | C | C | C | C | N | N | – |
| Trichloroacetic, 10% | N | C | – | C | C | C | C | – |
| Alkalides | ||||||||
| Ammonium Hydroxide, 25% | C | C | C | C | C | C | L | N |
| Sodium Hydroxide 3 Normal | C | C | C | N | C | N | L | N |
| Alcohols | ||||||||
| Amyl Alcohol, Butanol | C | C | L | C | C | C | C | N |
| Benzyl Alcohol | C | C | C | C | N | L | C | N |
| Ethanol, 70% | C | C | C | C | L | C | C | C |
| Ethanol, 98% | C | C | C | C | C | C | C | C |
| Ethylene Glycol | C | C | C | C | C | C | C | C |
| Glycerol | C | C | C | C | C | C | C | C |
| Isopropal, n-Propanol | C | C | C | C | C | C | C | C |
| Methanol, 98% | C | C | C | C | C | C | C | N |
| Propylene Glycol | C | C | C | C | C | L | C | C |
| Hydrocarbons | ||||||||
| Hexane | C | C | C | L | N | C | C | C |
| Xylene | C | C | C | L | N | C | C | C |
| Toluene, Benzene | C | C | C | L | N | C | C | C |
| Halogenated hydrocarbon | ||||||||
| Carbon Tetrachloride | C | C | C | L | N | L | C | C |
| Chloroform | C | C | C | L | N | N | C | C |
| Methylene Chloride | L | C | C | L | N | N | C | C |
| Monochlorobenzene, Freon | C | C | C | L | L | C | C | C |
| Trichloroethylene | C | C | C | L | N | C | C | C |
| Ketones | ||||||||
| Acetone | C | C | C | C | N | N | C | C |
| Cyclohexanone | C | C | C | C | N | N | C | N |
| Isopropylacetone | C | C | N | – | N | C | – | C |
| Methyl Ethyl Ketone | C | C | L | C | N | L | C | N |
| Methyl Isobutyl Ketone | C | C | N | L | N | C | C | C |
| Esters | ||||||||
| 2-Ethoxylate Acetate | L | L | L | – | N | L | C | L |
| Amyl Acetate | C | C | C | L | N | L | C | N |
| Benzyl Benzoate | C | C | – | – | N | C | C | C |
| Butyl Acetate | C | C | C | L | N | L | C | N |
| Ethyl acetate | C | C | C | L | N | N | C | N |
| Isopropyl Myristate | C | C | – | – | N | C | C | C |
| Methyl Acetate | L | C | L | L | N | N | C | L |
| Methyl Cellulose Acetate | L | N | C | L | N | N | C | L |
| Propyl Acetate | L | C | – | L | N | N | C | L |
| Propylene Glycol Acetate | C | C | C | C | N | N | C | C |
| Tricresyl Phosphate | – | C | – | – | N | C | C | – |
| Oxides – Ethers | ||||||||
| Acetonitrile (Methyl Cyanide) | C | C | C | C | N | N | C | C |
| Aniline | L | C | C | L | N | N | C | L |
| Diethyl Acetamide | C | C | C | – | – | N | C | C |
| Dimethyl Formamide | C | C | N | C | N | N | L | C |
| Dimethyl Sulfoxide (DMSO) | L | C | C | C | N | N | L | N |
| Dioxane | C | C | L | C | N | N | L | C |
| Ethyl Ether | C | C | C | L | C | L | C | C |
| Isopropyl Ether | C | C | N | C | C | C | – | C |
| Pyridine | C | C | L | L | N | N | C | C |
| Solvents with Nitrogen | ||||||||
| Tetrahydrofuran | C | C | L | C | N | N | L | N |
| Triethanolamine | C | C | C | – | – | C | C | C |
| Miscellaneous | ||||||||
| Formaldehyde Solution, 30% | C | C | C | C | C | L | L | C |
| Hydrogen Peroxide, 30% | N | C | C | C | C | C | C | C |
| Phenol, Aqueous, 10% | N | C | L | C | N | N | C | C |
| Silicone Oil & Mineral Oil | C | C | C | C | C | C | C | C |
Trong đó:
C: Lọc tốt
L: Hạn chế
N: Không lọc được
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